Reporter

Part:BBa_K389401:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

Sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 2639
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 818
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 2363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2545


Design Notes

arabinose promoter removed, GFP read-out exchanged with luciferase compared to the original part BBa_I746370

Source

  • luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
  • sensitivity tuner part consists inter alia of
    • ogr activator from phage P2 (BBa_I746350) without RBS (cf. Phyiscal DNA)
    • PF promoter from phage P2 (BBa_I746360)

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]